Nicotinamide-adenine dinucleotide phosphate, reduced Singlet oxygen

Peters, G.; Rodgers, M.A.J.
Biochim. Biophys. Acta 637, 43-52 (1981)

Reaction: NADPH + ¹O₂^* ® products and/or physical quenching
Solvent: CH₃CN, 80%, D₂O, 20%

k = 1.3 × 10⁸(L mol^-1 s^-1)

Experimental method: Flash photolysis
Analytical method: vis-UV absorption
Data type: Absolute value measured directly using indicator
Excitation wavelength: 337 nm

Photosensitizer = 2'-Acetonaphthone; Indicator = 1,3-Diphenylisobenzofuran. Added solute (0-20)e-5. [Sensitizer] = 1.0 × 10^-3 (mol L^-1). [Indicator] = 5.9 × 10^-5 (mol L^-1).

Peters, G.; Rodgers, M.A.J.
Biochim. Biophys. Acta 637, 43-52 (1981)

Reaction: NADPH + ¹O₂^* ® products
Solvent: CH₃CN, 20%, D₂O, 80%

k_react = 8.4 × 10⁷(L mol^-1 s^-1)

Experimental method: Flash photolysis
Analytical method: vis-UV absorption
Data type: Absolute value measured directly using indicator
Excitation wavelength: 337 nm

Photosensitizer = 2'-Acetonaphthone; Indicator = 1,3-Diphenylisobenzofuran; 63 ± 9% electron transfer giving O₂^-, detd. from buildup of radical anion in soln. contg. (2-20) × 10^-5 mol L^-1 1,4-benzoquinone. Added solute (0-20)e-5. [Sensitizer] = 1.0 × 10^-3 (mol L^-1). [Indicator] = 5.9 × 10^-5 (mol L^-1).