Nicotinamide adenine dinucleotide, reduced Singlet oxygen

Egorov, S.Yu.; Krasnovsky, A.A.
Sov. Plant Physiol. 33, 5-8 (1986)

Reaction: NADH + ¹O₂^* ® products and/or physical quenching
Solvent: D₂O

k = 2 × 10⁷(L mol^-1 s^-1)

Experimental method: Flash photolysis
Analytical method: infrared luminescence
Data type: Absolute value measured directly
Excitation wavelength: 337.1 nm

Photosensitizer = RF, water-sol. TPP derivs., or Chl a in micelles contg. Triton X-100.

Peters, G.; Rodgers, M.A.J.
Biochim. Biophys. Acta 637, 43-52 (1981)

Reaction: NADH + ¹O₂^* ® products and/or physical quenching
Solvent: CH₃CN, 75%, CH₃OH, 25%

k = 7.5 × 10⁷(L mol^-1 s^-1)

Experimental method: Flash photolysis
Analytical method: vis-UV absorption
Data type: Absolute value measured directly using indicator
Excitation wavelength: 337 nm

Photosensitizer = 2'-Acetonaphthone; Indicator = 1,3-Diphenylisobenzofuran. Added solute (0-80)e-5. [Sensitizer] = 1.0 × 10^-3 (mol L^-1). [Indicator] = 2.5 × 10^-5 (mol L^-1).

Peters, G.; Rodgers, M.A.J.
Biochim. Biophys. Acta 637, 43-52 (1981)

Reaction: NADH + ¹O₂^* ® products
Solvent: CH₃CN, 20%, D₂O, 80%

k_react = 4.3 × 10⁷(L mol^-1 s^-1)

Experimental method: Flash photolysis
Analytical method: vis-UV absorption
Data type: Absolute value measured directly using indicator
Excitation wavelength: 337 nm

Photosensitizer = 2'-Acetonaphthone; Indicator = 1,3-Diphenylisobenzofuran; 54.5 ± 15% electron transfer giving O₂^-, detd. from buildup of radical anion in soln. contg. (2-20) × 10^-5 mol L^-1 1,4-benzoquinone. Added solute 2.4 × 10^-5. [Sensitizer] = 1.6 × 10^-3 (mol L^-1). [Indicator] = 2.5 × 10^-5 (mol L^-1).

Peters, G.; Rodgers, M.A.J.
Biochim. Biophys. Acta 637, 43-52 (1981)

Reaction: NADH + ¹O₂^* ® products and/or physical quenching
Solvent: D₂O (mic)

k = 7.9 × 10⁷(L mol^-1 s^-1)

Experimental method: Flash photolysis
Analytical method: vis-UV absorption
Data type: Absolute value measured directly using indicator
Excitation wavelength: 337 nm

Photosensitizer = 2'-Acetonaphthone; Indicator = 1,3-Diphenylisobenzofuran; see also [80PET/ROD]. 0.1 mol L^-1 SDS. Added solute (0-80)e-5. [Sensitizer] = 1.0 × 10^-3 (mol L^-1). [Indicator] = 2.5 × 10^-5 (mol L^-1).

Vekshin, N.L.; Mironov, G.P.
Biophysics (Engl. Transl.) 26, 974-981 (1981)

Reaction: NADH + ¹O₂^* ® products and/or physical quenching
Solvent: H₂O

k ~ 2 × 10⁹(L mol^-1 s^-1), pH = 7.4

Experimental method: Photolysis
Analytical method: oxygen consumption
Data type: Relative value measured by steady state method

Photosensitizer = Flavine mononucleotide; Quencher = Azide ion, k = 2 × 10⁹ L/(mol·s). Use Azide ion as quencher.