2'-Deoxyguanosine Singlet oxygen

Devasagayam, T.P.A.; Steenken, S.; Obendorf, M.S.W.; Schulz, W.A.; Sies, H.
Biochemistry 30, 6283-6289 (1991)

Reaction: 2'-Deoxyguanosine + ¹O₂^* ® products and/or physical quenching
Solvent: D₂O

k = 5.2 × 10⁶(L mol^-1 s^-1), pH = 7.4, T = 310K

Experimental method: Chemical reaction
Analytical method: infrared luminescence
Data type: Derived from steady state measurements

Used solvent k_d = 1.9 × 10⁵ s^-1. ¹O₂* from NDPO₂.

Rougee, M.; Bensasson, R.V.
C.R. Seances Acad. Sci., Ser. II 302, 1223-1226 (1986)

Reaction: 2'-Deoxyguanosine + ¹O₂^* ® products and/or physical quenching
Solvent: D₂O

k = 5.0 × 10⁶(L mol^-1 s^-1), pH ~7, T = 293K

Experimental method: Flash photolysis
Analytical method: infrared luminescence
Data type: Absolute value measured directly
Excitation wavelength: 530 nm

Photosensitizer = 5,10,15,20-Tetrakis(4-sulfonatophenyl)porphine. Added solute 0.6-1.2 × 10^-3.

Midden, W.R.; Wang, S.Y.
J. Am. Chem. Soc. 105, 4129-4135 (1983)

Reaction: 2'-Deoxyguanosine + ¹O₂^* ® products
Reference Reaction: 2,5-Bis(hydroxymethyl)furan + ¹O₂^* ®
Solvent: H₂O

k/k_reference = 4.0 × 10^-1
pH = 10

Experimental method: Photolysis
Analytical method: HPLC
Data type: Relative value measured by steady state method

Photosensitizer = Methylene Blue; meas. k/k_ref = 0.4; sensitizer is immobilized on glass beads. Added solute 10^-4.