Cysteine Singlet oxygen

Michaeli, A.; Feitelson, J.
Photochem. Photobiol. 59, 284-289 (1994)

Reaction: CysSH + ¹O₂^* ® products and/or physical quenching
Solvent: D₂O, 75%, EtOH, 25%

k = 5 × 10⁷(L mol^-1 s^-1), T = 295K

Experimental method: Flash photolysis
Analytical method: infrared luminescence
Data type: Absolute value measured directly
Excitation wavelength: 532 nm

Photosensitizer = Rose Bengal dianion.

Devasagayam, T.P.A.; Sundquist, A.R.; Di Mascio, P.; Kaiser, S.; Sies, H.
J. Photochem. Photobiol., B 9, 105-116 (1991)

Reaction: CysSH + ¹O₂^* ® products and/or physical quenching
Solvent: D₂O

k = 8.3 × 10⁶(L mol^-1 s^-1), pH = 7.4, T = 310K

Experimental method: Chemical reaction
Analytical method: infrared luminescence
Data type: Derived from steady state measurements

used solvent k_d = 1.9 × 10⁴ s^-1; ¹O₂* from NDPO₂.

Di Mascio, P.; Devasagayam, T.P.A.; Kaiser, S.; Sies, H.
Biochem. Soc. Trans. 18, 1054-1056 (1990)

Reaction: CysSH + ¹O₂^* ® products
Solvent: CHCl₃, 50%, EtOH, 50%

k_react = 7.0 × 10⁷(L mol^-1 s^-1), T = 310K

Experimental method: Chemical reaction
Analytical method: chemical analysis
Data type: Derived from steady state measurements

used solvent k_d = 1 × 10⁵ s^-1; ¹O₂* from NDPO₂.

Di Mascio, P.; Devasagayam, T.P.A.; Kaiser, S.; Sies, H.
Biochem. Soc. Trans. 18, 1054-1056 (1990)

Reaction: CysSH + ¹O₂^* ® products and/or physical quenching
Solvent: CHCl₃, 50%, EtOH, 50%

k = 2.4 × 10⁸(L mol^-1 s^-1), T = 310K

Experimental method: Chemical reaction
Analytical method: infrared luminescence
Data type: Derived from steady state measurements

used solvent k_d = 1 × 10⁵ s^-1; ¹O₂* from NDPO₂.

Rougee, M.; Bensasson, R.V.; Land, E.J.; Pariente, R.
Photochem. Photobiol. 47, 485-489 (1988)

Reaction: CysSH + ¹O₂^* ® products and/or physical quenching
Solvent: D₂O

k £ 4 × 10⁴(L mol^-1 s^-1)

Experimental method: Flash photolysis
Analytical method: infrared luminescence
Data type: Derived from pH study

Photosensitizer = H₂TPPS^4- or H₂TMpyP⁴⁺; calcd. from k_obs = 8.9 × 10⁶ at pH 7 and pK_a(D₂O) = 8.6.

Egorov, S.Yu.; Krasnovsky, A.A.
Sov. Plant Physiol. 33, 5-8 (1986)

Reaction: CysSH + ¹O₂^* ® products and/or physical quenching
Solvent: D₂O

k = 1 × 10⁵(L mol^-1 s^-1)

Experimental method: Flash photolysis
Analytical method: infrared luminescence
Data type: Absolute value measured directly
Excitation wavelength: 337.1 nm

Photosensitizer = RF, water-sol. TPP derivs., or Chl a in micelles contg. Triton X-100.

Starostin, A.V.; Fedorovich, I.B.; Ostrovskii, M.A.
Biophysics (Engl. Transl.) 30, 1082-1088 (1985)

Reaction: CysSH + ¹O₂^* ® products and/or physical quenching
Solvent: EtOH

k = 8.0 × 10⁶(L mol^-1 s^-1)

Experimental method: Photolysis
Analytical method: electron spin resonance
Data type: Derived from steady state measurements
Excitation wavelength: 365 nm

Photosensitizer = (all-E)-Retinal; used solvent k_d = 3.5 × 10⁵ s^-1; obs. CysS·.