Trypsin Singlet oxygen

Shiryaev, V.M.; Fedorovich, I.B.; Sapezhinskii, I.I.; Khristophorov, V.L.; Kuz'min, V.I.; Smirnov, L.D.
Biophysics (Engl. Transl.) 25, 454-461 (1981)

Reaction: Trypsin + ¹O₂^* ® products and/or physical quenching
Solvent: H₂O

k = 8 × 10⁹(L mol^-1 s^-1), pH = 7.0, T = 298K

Experimental method: Photolysis
Analytical method: infrared luminescence
Data type: Derived from steady state measurements
Excitation wavelength: 365 nm

Photosensitizer = (all-E)-Retinal. used solvent k_d = 3.5 × 10⁵ s^-1.

Stevens, B.
Acc. Chem. Res. 6, 90-96 (1973)

Reaction: Trypsin + ¹O₂^* ® products and/or physical quenching
Solvent: H₂O

k = 7.1 × 10⁹(L mol^-1 s^-1), pH = 8.0, T = 288K

Experimental method: Photolysis
Analytical method: biological assay
Data type: Derived from steady state measurements

Photosensitizer = Methylene Blue and FMN; used solvent k_d = 5.0 × 10⁵ s^-1; calculated from data reported in [66GLA/SPI].