Subtilisin BPN' Singlet oxygen

Richard, P.; Blum, A.; Grossweiner, L.I.
Photochem. Photobiol. 37, 287-291 (1983)

Reaction: Subtilisin Novo + ¹O₂^* ® products
Solvent: D₂O

k_react = 3 × 10⁷(L mol^-1 s^-1), pH = 7.0

Experimental method: Photolysis
Analytical method: biological assay
Data type: Derived from steady state measurements

Photosensitizer = Hematoporphyrin IX; used solvent k_d = 1.5 × 10⁴ s^-1; used singlet oxygen quantum yield (HP) = 0.75. k_r = k for inactivation.