Luciferase Singlet oxygen

Thompson, A.; Nigro, J.; Seliger, H.H.
Biochem. Biophys. Res. Commun. 140, 888-894 (1986)

Reaction: Luciferase + ¹O₂^* ® products
Reference Reaction: DL-Histidine + ¹O₂^* ®
Solvent: H₂O

k_react = 1.9 × 10⁹(L mol^-1 s^-1), k_reference = 2.8 × 10⁷(L mol^-1 s^-1), pH = 8.0, T = 277K

Experimental method: Photolysis
Analytical method: biological assay
Data type: Relative value measured by steady state method

Photosensitizer = Rose Bengal dianion; sensitizer immobilized on glass beads.