b-Hydroxybutyrate dehydrogenase Singlet oxygen

Giulivi, C.; Sarcansky, M.; Rosenfeld, E.; Boveris, A.
Photochem. Photobiol. 52, 745-751 (1990)

Reaction: b-Hydroxybutyrate dehydrogenase + ¹O₂^* ® products and/or physical quenching
Reference Reaction: DL-Histidine + ¹O₂^* ®
Solvent: H₂O

k = 6.1 × 10⁸(L mol^-1 s^-1), k_reference = 1 × 10⁸(L mol^-1 s^-1), pH = 7.4

Experimental method: Photolysis
Analytical method: oxygen consumption
Data type: Relative value measured by steady state method
Excitation wavelength: 550 nm

Photosensitizer = Rose Bengal dianion; in submitochondria particles.

Robinson, R.S.; Roberts, A.J.; Campbell, I.D.
Photochem. Photobiol. 45, 231-234 (1987)

Reaction: b-Hydroxybutyrate dehydrogenase + ¹O₂^* ® products and/or physical quenching
Reference Reaction: DPBF + ¹O₂^* ®
Solvent: H₂O

k = 1.2 × 10⁹(L mol^-1 s^-1), pH = 7.2, T = 295K

Experimental method: Photolysis
Analytical method: vis-UV absorption
Data type: Derived from steady state measurements

Photosensitizer = Chloroaluminum(III) sulfophthalocyanine; estd. [¹O₂*] from DPBF consumption in MeOH; estd. rate in suspension of sub-mitochondria particles. [Sensitizer] = 1.5 × 10^-6 (mol L^-1).